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1996-03-04
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Document 0743
DOCN M9640743
TI Removal of human immunodeficiency virus by an 0.04-micron membrane
filter.
DT 9604
AU Oshima KH; Comans TW; Highsmith AK; Ades EW; Biological Products Branch,
Centers for Disease Control and; Prevention, Atlanta, Georgia 30333,
USA.
SO J Acquir Immune Defic Syndr Hum Retrovirol. 1995 Jan 1;8(1):64-5. Unique
Identifier : AIDSLINE MED/96142202
AB We tested the ability of a 0.04-micron nylon membrane filter to remove
human immunodeficiency virus (HIV) from tissue culture media containing
10% fetal calf serum. Endpoint titrations of infectious virus (ID50)
were performed on lymphocyte cultures. The presence of virus in the
cultures was determined using an enzyme-linked immunoabsorbant assay
(ELISA) of the HIV-1 P24 core antigen. In repeated experiments,
filtration of the virus suspension resulted in the removal of HIV below
detectable limits. The titer reduction was estimated to be greater than
8.5 x 10(2). These results suggest that this filter is effective in
removing HIV from fluids containing serum or serum products.
DE Culture Media Enzyme-Linked Immunosorbent Assay Filtration/METHODS
Human HIV Core Protein p24/ANALYSIS HIV-1/*ISOLATION & PURIF
Lymphocytes/VIROLOGY *Membranes, Artificial *Micropore Filters Nylons
Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S.
T-Lymphocytes/VIROLOGY JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).